Shalek and Love Lab researchers have increased the resolution on their signature high-throughput single-cell RNA-sequencing platform using a simple molecular biology trick known as "second-strand synthesis." The new and improved Seq-Well S3, described in Immunity, provides a five- and tenfold increase in identification of genes and RNA transcripts, respectively, and was applied to characterize various inflammatory skin conditions. The team is also using the technology to profile cancer cells, supported in part by the Bridge Project.