The KI’s Robert A. Swanson (1969) Biotechnology Center regularly adapts and evolves specialized techniques and technologies. In a cover-winning Structure paper, researchers from the Peterson (1957) Nanotechnology Materials Core Facility have developed a new 3D imaging workflow that integrates three imaging approaches to visualize the same sample at cryogenic temperature at different scales, providing a unique view into features of cell structure. Demonstrated in yeast, the process could be used for large-scale studies of frozen specimens in healthy, diseased, and therapeutic conditions. Currently, the research team is the only one in the US with the specific technological capabilities—volume cryo-focused ion beam scanning electron microscopy, cryo-fluorescence confocal microscopy, and transmission cryo-electron tomography—to run this entire workflow.