Instrumentation

Zeiss Microscope

Zeiss Microscope

Specifications

  • Zeiss Axioplan II upright microscope with motorized Z stage
  • EXFO-X-Cite fluorescent illumination source
  • Halogen transmitted light source
  • Objectives: 10X, 20X, 25X, 40X, 63X 1.3 NA and 100X-(0.9NA/ 1.4 NA)
  • Fluorescence filter cubes for DAPI, FITC, RD, Cy5
  • Hamamatsu Orca ER 1394 monochrome camera
  • QImaging color camera for histology
  • Openlab software for image acquisition and measurements
  • Volocity software for measurements and deconvolution

Filter cubes

Excitation/EmissionProbes/Fluorochromes
360/457 DAPI, Hoechst, Coumarin
488/528 FITC, Alexa488, GFP
555/617 Rhodamine, Alexa555, Texas Red, Phycoerythrin
647/685 Cy5, ALEXA 647