Instrumentation
Applied Precision DeltaVision Ultimate Focus Microscope with TIRF Module

Specifications
- Inverted Olympus X71 microscope with XYZ nano-motion stage
- Environmental chamber with temperature control and CO2 enrichment
- Solid state illumination (SSI) module coupled to a fiber optic cable
- 405 nm,488 nm, 512 nm, and 568 nm lasers
- Halogen transmitted light source
- Objectives: 10X, 20X, 40X, 60X, 100X
- Softworx software for image acquisition and deconvolution
- Photometrics CoolSNAP HQ camera
Fluorescence filter sets
| Excitation/Emission | Probes/Fluorochromes | Sample |
| 390/435 |
DAPI, Hoechst, Coumarin |
Fixed cells |
| 475/528 |
FITC, Alexa488, GFP |
Fixed/live cells |
| 542/585 |
Alexa546, Alexa 555, Rhodamine, Texas Red, Phycoerythrin |
Fixed/live cells |
| 632/685 |
Cy5, Alexa 647 |
Fixed/live cells |
| 438/465 |
CFP |
Live cells |
| 470/525 |
EGFP |
Live cells |
| 513/559 |
YFP |
Live cells |
| 575/630 |
Alexa595, mCherry |
Fixed/live cells |
Massachusetts Institute of Technology