Instrumentation

Applied Precision DeltaVision Ultimate Focus Microscope with TIRF Module

Applied Precision DeltaVision Ultimate Focus Microscope with TIRF module

Specifications

  • Inverted Olympus X71 microscope with XYZ nano-motion stage
  • Environmental chamber with temperature control and CO2 enrichment
  • Solid state illumination (SSI) module coupled to a fiber optic cable
  • 405 nm,488 nm, 512 nm, and 568 nm lasers
  • Halogen transmitted light source
  • Objectives: 10X, 20X, 40X, 60X, 100X
  • Softworx software for image acquisition and deconvolution
  • Photometrics CoolSNAP HQ camera

Fluorescence filter sets

Excitation/EmissionProbes/FluorochromesSample
390/435 DAPI, Hoechst, Coumarin Fixed cells
475/528 FITC, Alexa488, GFP Fixed/live cells
542/585 Alexa546, Alexa 555, Rhodamine, Texas Red, Phycoerythrin Fixed/live cells
632/685 Cy5, Alexa 647  Fixed/live cells
438/465 CFP Live cells
470/525 EGFP Live cells
513/559 YFP Live cells
575/630 Alexa595, mCherry Fixed/live cells