Instrumentation

Applied Precision DeltaVision Spectris Imaging System

Applied Precision DeltaVision Spectris Microscope

Specifications

  • Inverted Olympus X71 microscope with XYZ nano-motion stage
  • Environmental chamber with temperature control and CO2 enrichment
  • Mercury lamp illumination coupled to a fiber optic cable
  • Halogen transmitted light source
  • Objectives: 10X, 20X, 40X, 60X, 100X
  • Softworx software for image acquisition and deconvolution
  • Photometrics CoolSNAP HQ camera

Fluorescence filter sets

Excitation/EmissionProbes/FluorochromesSample
360/457 DAPI, Hoechst, Coumarin Fixed cells
488/528 FITC, Alexa488, GFP Fixed/live cells
555/617 Rhodamine, Alexa555, Texas Red, Phycoerythrin Fixed cells
647/685 Cy5, ALEXA 647  
436/465 CFP Live cells
470/525 EGFP Live cells
492/535 YFP Live cells
580/630 DsRed Express, DsRed2 Live cells

Massachusetts Institute of Technology