Instrumentation

Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope for Deep Tissue and Intravital Imaging

Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope

Specifications

  • Inverted Olympus IX81 microscope, 10X and 25X water immersion long working distance objectives
  • Olympus Z- drive: motorized focus/minimum increment: 0.01 µm
  • Motorized XY stage
  • >500 µm depth imaging  (depending on the optical property of the tissue)
  • Stage top CO2 incubator
  • Excitation: Laser- MaiTaiDeepSea HP: 690 nm - 1040 nm
  • Emission & Detector for MP imaging - 4 Photomultipliers/4 channel cubes
    • near UV filter (410-440 nm)
    • blue filter (457-487 nm)
    • green filter (502-548 nm)
    • red filter (572-642 nm)
  • Pixel size: 64x64 to 1024x1024
  • Scanning speed: (pixel time): 2 µs - 5 ms/High speed scanning mode: 16 frames/s (256x256)
  • Olympus acquisition software