Instrumentation

Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope for Deep Tissue Imaging

Specifications

• Inverted Olympus IX81 microscope, 25X and 60X water immersion long working distance objectives
• Olympus Z- drive: motorized focus/minimum increment: 0.01 µm
• >500 µm depth imaging  (depending on the optical property of the tissue)
• Stage top CO2 incubator
• Excitation: Laser- MaiTaiDeepSea HP: 690 nm - 1040 nm
• Emission & Detector for MP imaging - 2 Photomultipliers/2 channel cubes
  • green filter (495-540)
  • red filter (570- 630 nm)
• Pixel size: 64x64 to 1024x1024
• Scanning speed: (pixel time): 2 µs - 5 ms/High speed scanning mode: 16 frames/s (256x256)
• Olympus acquisition software