Instrumentation

Two Evo 150 Liquid Handling Decks In Baker Hood (HEPA) Enclosures

  • Small molecule library arraying and cherry picking
  • Automated cell plating, administration and removal of compounds via integrated automated CO2 incubator

Integrated Robotic Automated Assay Platform

  • Tecan Evo 150 liquid handling deck for compound and reagent delivery
  • BioTek 406 plate washer (cell plating and washing, ELISA-type assays)
  • Automated microplate centrifuge
  • Integrated with Tecan M1000 Plate Reader, Arrayscan Screening Microscope (see below for details)

Tecan M1000 Plate Reader

  • Flexible wavelength selection from UV to NIR
  • Absorbance, luminescence, fluorescence intensity (top and bottom reading), TRF, TR-FRET, fluorescence polarization
  • AlphaScreen and AlphaLISA technology
  • NanoQuant Plate compatibility for nucleic acid quantification

Arrayscan Screening Microscope With Confocal Capability

  • LED Light Engine designed for high performance excite blue (e.g. Hoechst), green (e.g. FITC, GFP), orange red (e.g. TRITC), deep red (e.g.. Texas Red), and long red (e.g. Cy5) fluorochromes
  • Nipkov spinning disk technology
  • User-definable z-range with 0.1μm resolution step size for confocal slices

Bl2+ Lentiviral shRNA Screening Platform

  • BL2+ cell culture hoods and 4 HeraCell incubators
  • Tecan Evo 150 liquid handling deck for cell plating and lentiviral manipulation
  • Enclosed robotics platform for packaging, manipulation and screening of lentiviral shRNA (RNAi) vectors integrated with a Liconic incubator for storage after cell plating