Facility Sponsors

Fannie E. Rippel Foundation

National Cancer Institute

ES Cell and Transgenics Facility

This shared resource of the Koch Institute provides fee-for-service support to all MIT investigators who utilize cultured ES cells or novel mouse models to study human diseases such as cancer. Access is available to all members of the MIT community, to the extent permitted by available capacity. Priority access is given to KI members, NCI-funded research projects and other contributing user groups in recognition of funding support.

Services Include:

  • Embryonic Stem Cell– *now offering modification by CRISPR/Cas9 in ES cells* 

    gene targeting, assistance with vector design, importation and testing of lines from outside sources, generation of feeders, generation of new ES lines from mouse strains and more.

  • Microinjection – mouse ES cells into blastocysts, and DNA, RNA or virus into pronucleus of fertilized mouse eggs.
  • Repository of Reagent Mice – containing Cre or FLP, and other useful strains.
  • Much more.

Services are scheduled on a first-come, first-served basis after approval.

Questions pertaining to our services?

Please email us. (escore@mit.edu)

 

The ES Cell & Transgenics Core is supported in part by funding provided to the Koch Institute from a National Cancer Institute Cancer Center Support Grant. We are thankful for the support that has been provided by the Fanny E. Rippel Foundation and by a grant from the National Institutes of Health to the Cell Migration Consortium.