Facility Sponsors

Fannie E. Rippel Foundation

National Cancer Institute

ES Cell and Transgenics Facility

This shared resource of the Koch Institute provides fee-for-service support to all MIT investigators who utilize cultured ES cells or novel mouse models to study human diseases such as cancer. Access is available to all members of the MIT community, to the extent permitted by available capacity. Priority access is given to KI members, NCI-funded research projects and other contributing user groups in recognition of funding support.

ES Cell Services Dynamic Graphic

Services Include:

  • Embryonic Stem Cell– genomic modification through locus targeting, assistance with vector designs, generation of new ES lines from mouse strains, importation and testing of lines from outside sources, generation of feeders, differentiation of ES lines into specific cell lineages or cell types and more.
  • Microinjection – Injection of mouse ES cells into blastocysts to generate chimeras and injection of DNA, RNA or virus into pronucleus of fertilized mouse eggs to generate transgenic mice.
  • Repository of Reagent Mice – containing Cre or FLP, and other useful strains.
  • Training and Troubleshooting for all aspects of Embryonic Stem Cell or primary cultures.
  • Much more.

Services are scheduled on a first-come, first-served basis after approval.

Questions pertaining to our services?

Please email us. (escore@mit.edu)

 

The ES Cell & Transgenics Core is supported in part by funding provided to the Koch Institute from a National Cancer Institute Cancer Center Support Grant. We are thankful for the support that has been provided by the Fanny E. Rippel Foundation and by a grant from the National Institutes of Health to the Cell Migration Consortium.