The Koch Institute features 12 core facilities that provide key technical services to our faculty. From routine (though essential) support services to advanced technical and consulting services, these cores facilitate and enhance the important research being conducted on a daily basis. Many of the facilities also offer training programs that enable Koch Institute staff, students, and postdoctoral fellows to acquire the additional technical and intellectual expertise needed to advance both their work and their careers.
The costs of these cores are partially defrayed by funds from a number of sources, most notably a Cancer Center Support Grant from the NCI to the KI. In recognition of this support, priority access is given to KI members, NCI-funded research projects and other contributing user groups. However, the KI is strongly committed to enabling biomedical research at MIT. Thus, to the extent permitted by available capacity, a number of the cores are accessible to the broader MIT community. See individual cores for details about access, user policies and service rates.
Learn about the people and projects at work by visiting the SBC Focus website.
The Applied Therapeutics & Whole Animal Imaging Core Facility assists and accelerates the process of moving scientific breakthroughs from the benchtops of the Koch Institute to the clinics for cancer patients. Our primary goal is to support and encourage the translational efforts using refined mouse models of human cancers to test
In addition to providing assistance with design, approval and execution of relevant preclinical trials, the facility also provides access to instrumentation for in vivo, whole animal imaging, including bioluminescence and microCT technologies. more >>
The Bioinformatics & Computing Core Facility provides Koch Institute researchers with assistance and training in a wide range of bioinformatics related topics. Examples include assistance with experimental design and subsequent analysis of next-generation sequencing (Illumina platform) and microarray experiments, genome annotation projects and other sequence and phylogenetic analysis applications. The Core also provides Koch Institute members with critical data backup as well as installation and maintenance of desktop hardware and a variety of software, including scientific applications. more >>
The Biopolymers & Proteomics Core Facility provides MIT researchers with integrated synthetic and analytical capabilities for biological materials, including DNA, proteins and nanoparticles. The Core encompasses a wide range of sophisticated technical expertise and state-of-the-art instrumentation.
This shared resource of the Koch Institute provides fee-for-service support to all MIT investigators who utilize novel mouse models to study human diseases such as cancer. The Rippel Mouse ES Cell & Transgenics Core Facility specializes in assisting researchers with the design, generation, maintenance, and analysis of mice containing designer mutations. We also provide skilled assistance with a variety of protocols utilizing mouse ES cells (establishment of new lines, differentiation into specific cell types).
The Flow Cytometry Core Facility provides high-speed cell sorting services as well as access, training, and support in the use of bench-top analysis flow cytometers. more >>
The Glassware Preparation Core Facility provides centralized services to all internal Koch Institute member laboratories, with the primary goal of supplying sterile glassware at an exceptionally high, tissue culture-grade standard. more >>
This Core facilitates the development of a wide range of experimental assays into high throughput screening strategies. Support for assay development includes liquid handling robotic systems, high content imaging capabilities, and management of RNAi and small molecule libraries. Also provides expert consultation for assay development and automation. Facility features multiple Tecan Evo liquid handlers, plate readers, and a confocal screening microscope. more >>
The Histology Core Facility assists investigators in producing quality histological slides from frozen, paraffin-embedded, and resin-embedded tissues, thus enabling investigators to better evaluate the pathologic consequences of various mutations or treatments. Services range from hematoxylin and eosin-stained and special stained slides, to advice and assistance in performing immunohistochemistry, immunofluorescence, and in-situ hybridization. more >>
The Media Preparation Core Facility provides internal Koch Institute member laboratories with a wide array of high-quality custom-made bacterial, yeast, and tissue culture media. more >>
The Microarray Technologies Core Facility is a shared institutional resource providing investigators with integrated facilities for microarray experiments on Affymetrix and Agilent platforms, including hybridization, scanning, processing and image analysis, and microarray validation tools such as RNA quality assessment and real time PCR. more >>
The Microscopy Core is a centralized facility housing state-of-the art imaging equipment, with a complete range of image acquisition and data analysis capabilities. Expert experimental advice, instrument training and access, and fee-based technical service are available for multiple imaging platforms:
This Core will provide support for new materials discovery and optimization, enabling development of drug- and gene-delivery vehicles, imaging, nano- and microparticles, and devices. Services will include in vivo testing in conjunction with the Applied Therapeutics & Whole Animal Imaging Core, as well as a range of tools for physical and chemical characterization of all types of nanoparticle systems. Contact Janeta Zoldan for more information.
The Shared Research Resources Core Facility oversees maintenance of shared research spaces (cold rooms, warm rooms, tissue culture rooms and darkrooms) and distributed equipment (autoclaves, ice machines, X-ray film developers, etc). The Core also coordinates implementation of the MIT Environment, Health & Safety management system (EHS-MS) by providing safety inspections, laboratory safety, staff training and hazardous waste management. Finally, the Core oversees dedicated KI research spaces developed to provide oversight of, and enable safe working practices with, high titer VSVg-pseudotyped viruses, high-risk isotopes, and the cesium γ-cell radiation source. Access is reserved for KI members.
Currently in development:
This Facility will support phage- and yeast-display screening technologies for peptide and antibody affinity binding.