Contact Information

Scott Manalis

room 76-261
phone (617) 253-5039
fax (617) 258-6264
email scottm@media.mit.edu

Manalis Lab

phone (617) 253-3203
fax (617) 253-5102
website

Administrative Assistant:

Mariann Murray
room 76-261
phone (617) 253-0632
email mariann@mit.edu

Related Items

Scott Manalis

Scott Manalis

Professor of Biological and Mechanical Engineering

Ph.D. 1998, Stanford University

 

KI Research Areas of Focus:
Detection + Monitoring

"Tumors are made up of single cells.  In aggregate, we know a great deal about the genetic and cellular defects that cause cancer, but we know comparatively little about the progress of individual cells. Our laboratory develops quantitative and real-time techniques for biomolecular detection and single cell analysis. We use conventional silicon processing techniques to fabricate fluidic devices, and exploit the unique physical properties associated with micro- and nanoscale dimensions for developing precision measurement methods. We have recently developed a technology that enables mass to be measured in the aqueous environment with a resolution that is a million-fold better than existing methods. We are able to use this technology to investigate how cell growth relates to progression through the division cycle, and if the response of cancer cells to pathway-directed therapeutics can be classified according to subtle changes in growth."

Dr. Manalis is a professor of biological and mechanical engineering at MIT and has been a faculty member at MIT since 1999. He was the recipient of the Presidential Early Career Award for Scientists and Engineers (PECASE) from the Department of Defense.  He has also been selected by Technology Review magazine as one of the 100 innovators under the age of 35.  Dr. Manalis received his B.S. degree in physics from the University of California at Santa Barbara in 1994, and his Ph.D. degree in applied physics from Stanford University in 1998.

Further Information

Research Summary

We have recently developed a technology that enables mass to be measured in the aqueous environment with a resolution that is a million-fold better than existing methods. This approach, known as the suspended microchannel resonator (SMR), places the fluid inside of the resonator instead of immersing the resonator in the fluid and thereby solves the long-standing problem of signal degradation from viscous drag. This has enabled single cells, nanoparticles and biomolecules to be weighed in solution with femtogram resolution.

We are currently exploring a wide range of biological applications with the SMR. For example, we are using the SMR's ability to resolve mammalian cell mass with a precision near 0.01% to investigate how cell growth relates to progression through the division cycle, and if the response of cancer cells to pathway-directed therapeutics can be classified according to subtle changes in growth.

We are also developing high performance fluidic interfaces to micro- and nanofluidic sensors such as the SMR. These interfaces utilize novel Teflon valves and pumps that are resistant to virtually all chemicals. Our microfluidic Autosampler Chip is capable of automating all fluidic manipulations necessary for performing precision measurements with the SMR sensor.

Selected References

M. Godin, F.F. Delgado, S. Son, W.H. Grover, A.K. Bryan, A. Tzur, P. Jorgensen, K. Payer, A.D. Grossman, M.W. Kirschner, S.R. Manalis. Using buoyant mass to measure the growth of single cells, Nature Methods, 7 387 (2010).

A.K. Bryan, A. Goranov, A. Amon, S.R. Manalis. Measurement of Mass, Density, and Volume During the Cell Cycle of Yeast, Proceedings of the National Academy of Sciences, 107 999 (2010).

T.M. Squires, R.J. Messinger, S.R. Manalis. Making it stick: convection, reaction, and diffusion in surface based biosensors, Nature Biotechnology, 26 417 (2008).

T.P. Burg, M.Godin, W. Shen, G. Carlson, J.S. Foster, K. Babcock, and S.R. Manalis. Weighing of Biomolecules, Single Cells, and Single Nanoparticles in Fluid, Nature , 446 1066 (2007).

Search PubMed for Manalis lab publications