The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope for Deep Tissue and Intravital Imaging

Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope

Specifications

  • Inverted Olympus IX81 microscope, 10X and 25X water immersion long working distance objectives
  • Olympus Z- drive: motorized focus/minimum increment: 0.01 µm
  • Motorized XY stage
  • >500 µm depth imaging  (depending on the optical property of the tissue)
  • Stage top CO2 incubator
  • Excitation: Laser- MaiTaiDeepSea HP: 690 nm - 1040 nm
  • Emission & Detector for MP imaging - 4 Photomultipliers/4 channel cubes
    • near UV filter (410-440 nm)
    • blue filter (457-487 nm)
    • green filter (502-548 nm)
    • red filter (572-642 nm)
  • Pixel size: 64x64 to 1024x1024
  • Scanning speed: (pixel time): 2 µs - 5 ms/High speed scanning mode: 16 frames/s (256x256)
  • Olympus acquisition software