The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Swanson Biotechnology Center

The Koch Institute features 12 core facilities that provide key technical services to our faculty. From routine (though essential) support services to advanced technical and consulting services, these cores facilitate and enhance the important research being conducted on a daily basis. Many of the facilities also offer training programs that enable Koch Institute staff, students, and postdoctoral fellows to acquire the additional technical and intellectual expertise needed to advance both their work and their careers.

The costs of many of these cores are partially defrayed by funds from a number of sources, most notably a Cancer Center Support Grant from the NCI to the KI. In recognition of this support, priority access is given to KI members, NCI-funded research projects and other contributing user groups. However, the KI is strongly committed to enabling biomedical research at MIT. Thus, to the extent permitted by available capacity, a number of the cores are accessible to the broader MIT community. See individual cores for details about access, user policies and service rates.

Learn about the people and projects at work by visiting the SBC Focus website


Biopolymers & Proteomics

The Biopolymers & Proteomics Core Facility provides MIT researchers with integrated synthetic and analytical capabilities for biological materials, including proteins, small molecules, and nanoparticles. The Core encompasses a wide range of sophisticated technical expertise and state-of-the-art instrumentation.

Overview of services

  • mass spectrometry-based proteomics approaches for identification, characterization, or quantitation of proteins from simple to complex mixtures
  • MALDI-TOF mass spectrometry
  • peptide synthesis and purification, including peptide arrays
  • high-pressure liquid chromatographic analysis and purification


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Flow Cytometry

The Flow Cytometry Core Facility provides high-speed cell sorting services as well as access, training, and support in the use of bench-top analysis flow cytometers. more >>

Flow Cytometry: Nanowell Cytometry

The Nanowell Cytometry platform is a shared resource housed in the Flow Cytometry Core that provides access to Microengraving and SeqWell technologies. Microengraving enables measurement of multiple traits from individual cells derived from a wide array of tissues or cell types, and the Core offers access to tools which enable isolation of single cells using an ultra-precise robotic micromanipulator. Seq-Well is a portable, low-cost platform for massively-parallel single-cell RNA-Seq. more >>

Histology

The Hope Babette Tang (1983) Histology Core Facility assists investigators in producing quality histological slides from frozen, paraffin-embedded, and resin-embedded tissues, thus enabling investigators to better evaluate the pathologic consequences of various mutations or treatments. Services include state-of-the art technical assistance and training in tissue sectioning, slide preparation and analysis, including automated immunohistochemistry and special staining, as well as access to expert pathology consultation. more >>

High Throughput Sciences

The High Throughput Sciences Core provides automated screening capabilities, access to curated small molecule and lentiviral shRNA (RNAi) libraries, assay development and screening services in both BL2 and BL2+ space, as well as consultation and training for researchers with specific needs or challenging problems.  more >>

Integrated Genomics & Bioinformatics

The Integrated Genomics and Bioinformatics Core is comprised of the Genomics Facility and the Barbara K. Ostrom Bioinformatics Facility. Together, these shared institutional resources provide Koch Institute investigators with support throughout all phases of modern genomics experimentation. more >>

Microscopy

The Microscopy Core Facility provides experimental consultation, fee-for-service imaging assistance, and training in and access to several imaging platforms, image acquisition equipment, and data analysis software packages.

Services include:

  • light and epifluorescence microscopy
  • deconvolution-based microscopy for sensitive, high-resolution fluorescence imaging
  • 3D structured illumination microscopy
  • spinning disk confocal microscopy
  • scanning confocal microscopy
  • multiphoton scanning confocal microscopy and intravital imaging
  • total internal reflection fluorescence (TIRF) microscopy
  • advanced fluorescence microscopy (FRAP- fluorescence recovery after bleaching, FRET- fluorescence resonance energy transfer, photo-recovery)
  • laser capture microdissection

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Nanotechnology Materials

The Peterson (1957) Nanotechnology Materials Core Facility was established by Thomas F. Peterson, Jr. (1957) to assist investigators in research relating to nanomaterials.

The Core offers a broad range of equipment and expertise to work with nanomaterials for the purposes of both characterization and imaging.   Core imaging capabilities include a high-performance field emission transmission electron microscope (equipped with STEM, EELS, EDS and cryo-imaging), high performance field emission scanning electron microscope and focused ion beam (equipped with STEM and cryo-imaging), a cryo-fluorescent confocal microscope for CLEM workflows, and an atomic force microscope (equipped with a liquid cell). Instrumentation for material characterization includes high throughput dynamic light scattering, nanoparticle sizing and counting, and rheometry.  more >>

Preclinical Modeling, Imaging & Testing

The Preclinical Modeling, Imaging & Testing (PMIT) Core’s mission is to navigate and streamline the path from the development of new therapeutic, diagnostic and basic discovery biomedical tools at the bench to their evaluation within a valid biological system. The PMIT Core combines the expertise and instrumentation to facilitate the generation and high-resolution characterization of disease models, to assist investigations of the mechanisms of disease development, to enable the evaluations of drug efficacy and resistance, and to provide the means for testing novel strategies and devices for disease detection and drug delivery. more >>

Zebrafish

The Zebrafish Core Facility supports researchers in the use of zebrafish as a model organism by providing expert experimental support as well as the expertise, aquatic systems and other infrastructure for maintaining a healthy breeding colony.  The facility offers to meet many needs, ranging from experiments with limited scope and duration, to long-term maintenance of dozens of transgenic and/or mutant lines. more >>

Glassware Preparation

The Glassware Preparation Core Facility provides centralized services to internal Koch Institute member laboratories, with the primary goal of supplying sterile glassware at an exceptionally high, tissue culture-grade standard. more >>

Media Preparation

The Media Preparation Core Facility provides internal Koch Institute member laboratories with a variety of high-quality custom-made bacterial, yeast, and tissue culture media. more >>

Shared Research Resources

The Shared Research Resources Core Facility oversees maintenance of shared research spaces (cold rooms, warm rooms, tissue culture rooms and darkrooms) and distributed equipment (autoclaves, ice machines, X-ray film developers, etc). The Core also coordinates implementation of the MIT Environment, Health & Safety management system (EHS-MS) by providing safety inspections, laboratory safety, staff training and hazardous waste management. Finally, the Core oversees dedicated KI research spaces developed to provide oversight of, and enable safe working practices with high-risk isotopes, and the cesium γ-cell radiation source. Access is reserved for KI members.

Facility Sponsors