The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Services

Platform overview and consultation

Potential new users are provided an overview of how the platform works and basic methodologies used in the lab.

All prospective users begin with a consultation to discuss their project plan and identify feasibility for use of the platform. Contact Lead Microengraving Specialist Shanu Mehta to schedule a consultation.

Nanowell devices

Users may purchase nanowell devices for use in their laboratories. 

  • PDMS Standard configuration (1”x3” slide) for microengraving or imaging
  • SeqWell array + membrane: includes a functionalized array and membrane; no beads (most common)
  • Ready to use SeqWell kits: includes a functionalized array and membrane, loaded with oligo beads
  • Functionalized SeqWell array: includes a functionalized array only; no membrane or beads (requires plasma cleaner)

SeqWell sample processing

The Core offers training and assisted use of the SeqWell platform. All users must set up a complimentary initial consultation to discuss project scope and feasibility for platform use. Contact Shanu Mehta to set up a consultation.

Single-cell retrieval

We use a robotic micromanipulator (ALS CellCelector) for robust, high throughput single cell picking. The system offers bright field and fluorescence channels (DAPI, APC, PE), to aid in isolation based on expression of fluorescence markers or cell morphology. We offer in-depth user training for independent use of the system as well as fully staff-assisted use of equipment. (More details on equipment can be found here).

Microengraving

Users have access to the entire Microengraving process, materials and equipment. We offer in-depth training for users to learn each step of the technique or offer services to run the experiment with our expertise.

Single-cell imaging (12+ fluorescence channels)

PicoCell offers multi spectral fluorescence imaging. We offer in-depth training for users for independent equipment use, as well as fully staff-assisted use of equipment.

For pricing and an explanation of how fees are set, see pricing page.

To request a service, please see Core Access page.