The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Introduction to Nanowell Cytometry

What is a nanowell device?

An array of wells, manufactured from PDMS (a silicone-based organic polymer), using photolithography and replica molding. The device is comprised of blocks of subnanoliter wells within a 1” X 3” glass slide. Each array has roughly 85,000 wells, which can hold 1 to 3 cells each.

 

Complete Array Wells

 

What is microengraving?

A process in which cells are confined within a nanowell device and sealed against a functionalized glass slide to study the function and phenotype of the cells within the wells based on individual single cell secreted proteins.

Review article on Microengraving

A 7 * 7 block showing different phenotypes of cells upon microengraving

What is nanowell cytometry?

The measurement of characteristics of cells that are contained within a nanowell device. The measurement is done via multispectral fluorescence imaging.

A 7 * 7 grid showing different phenotypes of cells in wells of a nanowell device.

Information on Love Laboratory

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