What is a nanowell device?
An array of wells, manufactured from PDMS (a silicone-based organic polymer), using photolithography and replica molding. The device is comprised of blocks of subnanoliter wells within a 1” X 3” glass slide. Each array has roughly 85,000 wells, which can hold 1 to 3 cells each.
What is microengraving?
A process in which cells are confined within a nanowell device and sealed against a functionalized glass slide to study the function and phenotype of the cells within the wells based on individual single cell secreted proteins.
A 7 * 7 block showing different phenotypes of cells upon microengraving
What is nanowell cytometry?
The measurement of characteristics of cells that are contained within a nanowell device. The measurement is done via multispectral fluorescence imaging.
A 7 * 7 grid showing different phenotypes of cells in wells of a nanowell device.