The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Services

Full Service (Assisted basis)

  • High resolution analytical TEM imaging for chemical analysis using EDS, EELS and STEM.
  • Cryo-TEM imaging for elucidating the high resolution structure of radiation-sensitive specimens or cryo-preserved hydrated specimens.
  • SEM/FIB and cryo-SEM/FIB imaging with a focus on biologically relevant samples, including STEM and cryo-STEM analysis.
  • Cryo-fluorescence imaging.
  • CLEM - Correlative workflows between fluorescence and electron microscopy imaging.
  • Conventional chemical fixation and resin embedding of biological samples for EM.
  • Immuno-gold labeling.
  • Ultramicrotome and cryo-ultramicrotome sectioning.
  • Cryo sample preparation including: ethane plunge freezing, high pressure freezing, freeze fracture, freeze substitution.

Self Service (24/7 access)

  • DLS: particle size characterization of biological macromolecular assemblies and nanoparticles from 0.5 – 1000 nm.
  • Nanoparticle counting and sizing.
  • Rheometer: analysis of liquid, suspension, slurry, or film specimen response to applied forces. Accessories include high temp oven, UV curing plate, and DMA tension clamp.
  • AFM: Specimen surface imaging and quantitative nanomechanical (QNM) characterization. Includes access to a liquid cell for measurements on hydrated samples.
  • High resolution SEM/FIB imaging of specimens with 3D reconstruction capability on the subcellular level.
  • High resolution TEM imaging.