The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Methods

Users are encouraged to consult with Core staff in all microscopy methods.

Users are expected to be familiar with basic immunostaining and fixation techniques and slide preparation. A common method to prepare samples is available here.

The Fluorescent Protein database has several useful tools for choosing the right fluorophore for your experiment. It includes a spectra viewer, FRET efficiency calculators, and microscope fluorophore efficiency reports. We will be adding KI-MIT microscope configurations to the database over time.

Additional up-to-date methodology can be found in useful links.