Users are encouraged to consult with Core staff in all microscopy methods.
Users are expected to be familiar with basic immunostaining and fixation techniques and slide preparation. A common method to prepare samples is available here.
The Fluorescent Protein database has several useful tools for choosing the right fluorophore for your experiment. It includes a spectra viewer, FRET efficiency calculators, and microscope fluorophore efficiency reports. We will be adding KI-MIT microscope configurations to the database over time.
Additional up-to-date methodology can be found in useful links.