The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

Zeiss Microscope

Zeiss Microscope

Specifications

  • Zeiss Axioplan II upright microscope with motorized Z stage
  • EXFO-X-Cite fluorescent illumination source
  • Halogen transmitted light source
  • Objectives: 10X, 20X, 25X, 40X, 63X 1.3 NA and 100X-(0.9NA/ 1.4 NA)
  • Fluorescence filter cubes for DAPI, FITC, RD, Cy5
  • Hamamatsu Orca ER 1394 monochrome camera
  • QImaging color camera for histology
  • Openlab software for image acquisition and measurements
  • Volocity software for measurements and deconvolution

Filter cubes

Excitation/EmissionProbes/Fluorochromes
360/457 DAPI, Hoechst, Coumarin
488/528 FITC, Alexa488, GFP
555/617 Rhodamine, Alexa555, Texas Red, Phycoerythrin
647/685 Cy5, ALEXA 647