The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

Applied Precision DeltaVision Ultimate Focus Microscope with TIRF Module

Applied Precision DeltaVision Ultimate Focus Microscope with TIRF module

Specifications

  • Inverted Olympus X71 microscope with XYZ nano-motion stage
  • Environmental chamber with temperature control and CO2 enrichment
  • Solid state illumination (SSI) module coupled to a fiber optic cable
  • 405 nm,488 nm, 512 nm, and 568 nm lasers
  • Halogen transmitted light source
  • Objectives: 10X, 20X, 40X, 60X, 100X, 100X TIRF
  • Softworx software for image acquisition and deconvolution
  • Photometrics CoolSNAP HQ camera

Fluorescence filter sets

Excitation/EmissionProbes/FluorochromesSample
390/435 DAPI, Hoechst, Coumarin Fixed cells
475/528 FITC, Alexa488, GFP Fixed/live cells
542/585 Alexa546, Alexa 555, Rhodamine, Texas Red, Phycoerythrin Fixed/live cells
632/685 Cy5, Alexa 647  Fixed/live cells
438/465 CFP Live cells
470/525 EGFP Live cells
513/559 YFP Live cells
575/630 Alexa595, mCherry Fixed/live cells