The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

Applied Precision DeltaVision-OMX Super-Resolution Microscope

Applied Precision DeltaVision-OMX Super-Resolution Microscope

Specifications

  • Experimental microscope (OMX) that utilizes 3 Dimensional Structured Illumination (3D-SIM) which offers eight (8) fold improvement in volume resolution compared to classical microscopes
  • 3 Dimensional Structured Illumination Microscopy (3D-SIM) Imaging
    • X, Y resolution between 80-120 nm
    • Z resolution between 250-350 nm (depending on wavelength)
    • >10 micron depth imaging (depending on spherical correction)
  • Imaging: 100X objective, simultaneous imagingon three (3) EM-CCD cameras
  • Widefield illumination for fast conventional / live cell imaging
  • Softworx processing software

 

Excitation/EmissionProbes/FluorochromesSample
405/435 DAPI, Hoechst, Coumarin Fixed cells
488/528 FITC, Alexa488, GFP Fixed/live cells
594/630 Alexa 568, Alexa595, Rhodamine, Texas Red Fixed/live cells