The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

Olympus FV1200 Laser Scanning Confocal for live and thick section imaging

FV1200

Specifications

  • Inverted Olympus IX83 microscope, 4X and 10X air objectives and 30X and 60X silicon oil long working distance objectives
  • Stage incubator with heat and CO2
  • Olympus Z- drive: motorized focus/minimum increment: 0.01 µm
  • Motorized XY stage
  • Lasers: 405 nm, 440 nm, 473 nm, 559 nm, and 635 nm
  • 3 PMTs and 2 High Sensitivity Detectors
  • Filter cubes for PMTs: 430-455 nm, 465-495 nm, 490-540 nm, and 575-675 nm
  • Filter cubes for High Sensitivity Detectors:
  • Pixel size: 64x64 to 1024x1024
  • Scanning speed: (pixel time): 2 µs - 5 ms/High speed scanning mode: 16 frames/s (256x256)
  • Olympus acquisition software
  • Hamamatsu ImagEM high sensitivity camera for imaging bioillumination.