The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

Nikon A1R Ultra-Fast Spectral Scanning Confocal Microscope

Nikon 1AR Ultra-Fast Spectral Scanning Confocal Microscope

Specifications

  • Inverted Ti Nikon microscope, 10X, 20X, 40X water immersion, and 60X and 100X oil immersion objectives
  • Nikon XYZ Motorized stage
  • Perfect Focus with Motorized Nosepiece
  • Environment chamber with temperature control and CO2 enrichment
  • Nikon A1- SHR High Speed Scan Head & Controller (Resonant Scanner)
  • Lasers: 405 nm, 457 nm, 488 nm, 561 nm, 640nm
  • 4 Standard Photomultipliers (PMT) detectors
  • Standard Detector Filters: 450/50 nm, 485/40 nm, 525/50 nm, 545/40 nm  and 600/50 nm
  • Nikon A1 Spectral Detector (32 PMT array; wavelength range: 400-770 nm)
  • Lasers: 405 nm, 457 nm, 477 nm, 488 nm, 514 nm, 561 nm, 640 nm
  • Lumen Fluorescence Illumination Source
  • Standard Image Acquisition
    • Pixel – 4096X4096 maximum
    • Speed  - Standard 1 frame/sec (512X512)
  • High Speed Image Acquisition:
    • X-axis resonant scanner
    • Speed- Standard 30 frames/sec (512X512)
    • Highest speed 230 frames/second (512X64)
  • Nikon NIS Elements Acquisition Software