The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Instrumentation

To determine which fluorophores may be run on each instrument, consult this document.

Benchtop Analyzers

FACSCANs

FACSCAN

  • FACScan-1: 1 laser, 3 color analyzer with 488 excitation, running CellQuest Pro on Mac OSX
  • FACScan-2: 1 laser, 3 color analyzer with 488 excitation, running CellQuest Pro on Mac OSX

FACSCALIBURs

FACSCALIBUR

  • FACS Calibur-1: 2 laser, 4 color analyzer, running CellQuest Pro on MacOSX
  • FACS Calibur HTS-2: 2 laser, 4 color analyzer running CellQuest Pro on MacOSX, equipped with HTS (high throughtput sampler), which will load samples from a 96 well plate

FACS LSRs

FACS LSR

  • FACS LSR II HTS-1: 4 laser, 10 color analyzer running BD FACSDIVA software on XP, also equipped with HTS
  • FACS LSR II HTS-2: 4 laser, 11 color analyzer running BD FACSDIVA software on XP, also equipped with HTS
  • LSR FORTESSA HTS: 4 laser, 14 color analyzer running BD FACSDIVA and equipped with HTS

FACSCANTO

FACSCANTO

  • FACS Canto II HTS-1: 3 laser, 8 color analyzer, running BD FACSDIVA software on XP, also equipped with HTS

Cell Sorters

MOFLOs

MOFLO

  • MoFlo2: 5 laser, 7 color sorter running Summit software on XP. Can sort into 1.5 mL, 5 mL, 15 mL or 50 mL tubes. Up to 2 populations can be sorted at one time.
  • MoFlo3: 5 laser, 7 color sorter running Summit software on XP. Can sort into 1.5 mL, 5 mL, 15 mL or 50 mL tubes. Can sort into 96 well plates. Up to 2 populations can be sorted at one time.

FACS ARIAs

FACS ARIA

  • Aria1: 4 laser, 11 color sorter running BDFACS Diva software on XP. Can sort into 1.5 mL, 5 mL, 15 mL, or 96 well plates. Can sort up to 4 populations.
  • Aria2: 2 laser, 7 color sorter running BDFACS Diva software on XP. Can sort into 1.5 mL, 5 mL, 15 mL, or 96 well plates. Can sort up to 4 populations.
  • Aria3: 4 laser, 11 color sorter running BDFACS Diva software on XP. Can sort into 1.5 mL, 5 mL, 15 mL, or 96 well plates. Can sort up to 4 populations at one time.