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Please note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Current rates effective July 1, 2020.
Each service is described in detail on the Services page.
The Preclinical Modeling Facility uses iLab, an online system, to streamline the process of requesting and billing core services. Information on registering for an iLab account is available on the Core Access page.
|Gene Targeting in ES cells||Gene targeting with 240-288 colonies isolated for screening||$2813||$5624|
|Small targeting, isolating 96 or fewer colonies||$1998||$3599|
|Assistance with transgenic vector design||$100/hr (2hr FREE)||$200/hr|
|Please start by reading our "Beginner’s Guide to Gene Targeting" on Methods|
Culture of ES cells and preparation for their injection into blastocysts
|Preparation of cocktail containing Cas9/sgRNA complex and HDR DNA for injection into zygotes||$243||$372|
Injection of DNA (BAC or Transgenic), CRISPR-RNPS, RNA or virus into pronucleus of fertilized mouse eggs (C57BL/6) or
Injection of ES cells into pre-implantation, diploid blastocysts (C57BL6)
|Karyotyping||Prepare and Ship 1 or 2 mouse ES lines for Karyotyping at Cell Line Genetics (CLG)||$365||$608|
|Each additional line added to a shipment to CLG||$122||$181|
|(Cell Line Genetics charges $450 per line for karyotyping)|
Cells that will be placed in a rodent must be free from known pathogens. Cell lines will be expanded according to testing requirments and submitted.
(Charge of $805 for testing at IDEXX, not included)
Pathogen-free mice are available for transfer to MIT Animal Facilities only, not to other institutions.
All mice are also availiable from The Jackson Laboratories. See Repository Mice Available page for details, including JAX strain#'s.
Training options include, but are not limited to:
Culture of mouse embryonic stem (ES) cells and organoids
Generation of new cell lines: ES cells, MEFs, mouse intestinal organoids or other primary cell cultures (all mammals)
Stable transfection of cell lines to introduce elements such as reporters
Isolation of mouse eggs, blastocysts or embryos
|Vials of 3x105 irradiated NeoR MEFs for use as feeders. One vial covers one multiwell plate or one 10cm dish.||$18||$27|
|Vial of germline-proven ES cells||$394||$563|
|Media, Serum, and Matrigel for specilized cell culture||at cost|
Other services such as cryopreservation of sperm and embryos, IVF, and genotyping are also available from DCM.
Please see the DCM website (Certificates Required) for more details.
*As workflow permits, we do provide services to non-MIT academic institutions and for-profit researchers.