The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together


Please note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Current rates effective July 1, 2016.

Each service is described in detail on the Services page.

The ES Cell & Transgenics Core uses iLab, an online system, to streamline the process of requesting and billing core services.  Information on registering for an iLab account is available on the Core Access page.

Service Description KI

MIT(non-KI)    external academic                    

Gene Targeting in ES cells Gene targeting with 240-288 colonies isolated for screening $2250 $4000             $4500
  Small targeting, isolating 96 or fewer colonies  $1600 $3000              $3200
  Assistance with transgenic vector design FREE $90/hr              $120/hr
  Please start by reading our "Beginner’s Guide to Gene Targeting" on Methods    

Injection of DNA (BAC, Transgenic or CRISPR), RNA or virus into pronucleus of fertilized mouse eggs (C57BL/6)

Requests for Pronucler injection (PNI) of DNA, RNA or virus are processed by DCM.   Please see the DCM website for the forms to request these services(MIT Certificates Required)


$2000              $2000

  For CRISPR injections, 20ug of Cas9 mRNA $60 $90                  $90
  Injection of ES cells into pre-implantation, diploid blastocysts (C57BL6) $1420 $2000             $2000
  Injection of F1 hybrid ES cells into CD-1 tetraploid blastocysts $2110 $2836             $2836
  Culture of ES cells and preparation for their injection into blastocysts $180 $255               $275
Karyotyping Prepare and Ship 1 or 2 mouse ES lines for Karyotyping at Cell Line Genetics (CLG) $300 $450               $500
  Each additional line added to a shipment to CLG $100 $150               $150
  (Cell Line Genetics charges $450 per line for karyotyping)    
Pathogen Tests ES cells imported from outside MIT will be expanded and submitted  (Charge of $575 for testing at IDEXX, not included) $180 $255               $275

Pathogen-free mice are available for transfer to MIT Animal Facilities only, not to other institutions.

All mice are also availiable from The Jackson Laboratories. See Repository Mice Available page for  details, including JAX strain#'s.

C57B6L/6j  $30/$45

129S4/Jae  $100/$150

TPA-/- (for NeorR MEFs) $100/$150

Per mouse

Training and Assisted Work

Training options include, but are not limited to:

$60/hr $90/hr              $120/hr

Culture of ES cells

Generation of MEFs

Isolation of mouse eggs or embryos 

Isolation and culture of blastocysts

Generation of new ES cell lines

Differentiation of ES cells to specific cell tissue type in vitro

Optimizing breeding conditions for mice

Reagents Vials of 3x10'6 irradiated NeoR MEFs for use as feeders. One vial covers one multiwell plate or one 10cm dish. $12 $18                $18
  Vial of p2 NeoR MEFs for expansion to generate your own stocks of feeders. $55 $82.50          $82.50  
  Vial of p2 DR4 MEFs (HygroR - PuroR- NeoR) $100 $150             $150
  Vial of germline-proven ES cells $350  $475            $500
  Media, Serum, and ESGRO for ES cell culture at cost

Other services such as cryopreservation of sperm and embryos, IVF, and genotyping are also available from DCM.

Please see the DCM website (Certificates Required) for more details.

*As workflow permits, we do provide services to non-MIT academic institutions and for-profit researchers.