The David H. Koch Institute for Integrative Cancer Research at MITThe David H. Koch Institute for Integrative Cancer Research at MIT

Massachusetts Institute of Technology

National Cancer Institute Cancer Center

Science + Engineering... Conquering Cancer Together

Methods

Preclinical testing: Designing an in-vivo study

Animal imaging: Visualizing structures and biological processes in vivo

  • Optical imaging
    • Fluorescence vs. bioluminescence
    • How to choose a dye
    • Minimize autofluorescent/autoluminescent background
    • Optimal settings (exposure time, pixel size, fstop, filters)
    • Advanced settings (spectral unmixing, tomography)
  • Magnetic resonance imaging
    • How does it work?
    • Pros & Cons
    • MRI, fMRI, MRS, DWI/DTI, x-nuclei
    • How to choose a pulse sequence
    • Image weighting and contrast
    • Contrast agents (Gd, iron oxide) vs. labels (fluorine)
    • Common pulse sequences
    • Useful links