Instrumentation

Nikon Spinning-disk Confocal Microscope with TIRF Module

Specifications

• Nikon Ti inverted microscope with XYZ motorized stage with environment chamber (temperature control and CO2 delivery)
• Perfect focus with motorized nose piece
• Innova 70C Spectrum Laser (488, 568 and 647 nm) with an Acusto Optical Tunable Filter (AOTF)
• McBain-Yokogawa spinning disk confocal head with fiber optic input
• Spectra 7 wavelengths solid state illumination fluorescence source
• Objectives: Nikon 4X, 10X DIC, 20X, 40X DIC, 60X DIC 1.4 NA, 100X DIC 1.4 NA, Apo TIRF 100X 1.45 NA, Apo TIRF 100X 1.49 NA
• Nikon TIRF back illuminator with fiber optic input
• Hamamatzu Orca ER camera for confocal imaging
• Photometrics CoolSnap HQ camera for TIRF and bright field imaging
• Nikon NIS Elements Acquisition software