Instrumentation

Applied Precision DeltaVision-OMX Super-Resolution Microscope

Applied Precision DeltaVision-OMX Super-Resolution Microscope

Specifications

  • Experimental microscope (OMX) that utilizes 3 Dimensional Structured Illumination (3D-SIM) which offers eight (8) fold improvement in volume resolution compared to classical microscopes
  • 3 Dimensional Structured Illumination Microscopy (3D-SIM) Imaging
    • X, Y resolution between 80-120 nm
    • Z resolution between 250-350 nm (depending on wavelength)
    • >10 micron depth imaging (depending on spherical correction)
  • Imaging: 100X objective, simultaneous imagingon three (3) EM-CCD cameras
  • Widefield illumination for fast conventional / live cell imaging
  • Softworx processing software

 

Excitation/EmissionProbes/FluorochromesSample
405/435 DAPI, Hoechst, Coumarin Fixed cells
488/528 FITC, Alexa488, GFP Fixed/live cells
594/630 Alexa 568, Alexa595, Rhodamine, Texas Red Fixed/live cells