Instrumentation
Applied Precision DeltaVision-OMX Super-Resolution Microscope
Specifications
- Experimental microscope (OMX) that utilizes 3 Dimensional Structured Illumination (3D-SIM) which offers eight (8) fold improvement in volume resolution compared to classical microscopes
- 3 Dimensional Structured Illumination Microscopy (3D-SIM) Imaging
- X, Y resolution between 80-120 nm
- Z resolution between 250-350 nm (depending on wavelength)
- >10 micron depth imaging (depending on spherical correction)
- Imaging: 100X objective, simultaneous imagingon three (3) EM-CCD cameras
- Widefield illumination for fast conventional / live cell imaging
- Softworx processing software
| Excitation/Emission | Probes/Fluorochromes | Sample |
|---|
| 405/435 |
DAPI, Hoechst, Coumarin |
Fixed cells |
| 488/528 |
FITC, Alexa488, GFP |
Fixed/live cells |
| 594/630 |
Alexa 568, Alexa595, Rhodamine, Texas Red |
Fixed/live cells |
Massachusetts Institute of Technology
