Instrumentation

Below is the basic information for all instruments in the Microscopy and Imaging Facility. Additional detailed and up-to-date information regarding instrument capabilities can be found here.

Fluorescence and Bright Field Microscopes

• Applied Precision DeltaVision Spectris Imaging System, best for live/fixed thin samples
  • Inverted Olympus X71microscope with XYZ nano-motion stage, Solent environment chamber, Mercury lamp illumination
  • Softworx deconvolution software
• Applied Precision DeltaVision Microscope with Ultimate Focus and TIRF module, best for live cell imaging, photokinetics experiments and TIRF imaging
  • Inverted Olympus X71 microscopes with XYZ nano-motion stage, environment chamber, Solid State Illumination module, four (4) lasers, Ultimate Focus, TIRF module
  • Softworx deconvolution software
• Applied Precision DeltaVision-OMX Super-Resolution Microscope, best for imaging thin samples
  • Experimental microscope (OMX) with XYZ nano-motion stage, Structured Illumination, three (3) lasers, three cameras, heated stage
  • Softworx processing software
• Nikon Spinning-disk Confocal Microscope with TIRF module, best for live imaging/thick samples and for live TIRF imaging
  • Nikon inverted microscope with XYZ stage, with lateral arm Yokogawa spinning disk and back illumination TIRF module, environment chamber, laser (3 lines) / 7 Wavelength Solid State Illumination
  • Nikon NIS Elements software
• Nikon 1AR Ultra-Fast Spectral Scanning Confocal Microscope, best for imaging live/fixed thick samples
  • Nikon inverted microscope with XYZ stage & environment chamber, a four standard detector unit and a 32 ultra fast spectral (resonant scanning) detector unit, 3 lasers (7 lines) and Lumen lllumination Source
  • Nikon NIS Elements software
• Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope, best for imaging live/fixed thick samples
  • Olympus inverted IX81 microscope with heated & CO2 stage incubator, MaiTai laser, photomultiplier detectors -2 channels
  • Olympus software.
• Zeiss microscope, good for imaging fixed thin samples and histology
  • Zeiss Axioplan II upright microscope with monochrome and color cameras, Mercury lamp illumination
  • OpenLab / Volocity software

Specialty Microscopes

• Olympus microscope specialized for Spectral Karyotyping (SKY) and Fluorescence In Situ Hybridization (FISH)
  • Olympus X70 upright microscope with SpectraCube interferometer, Xenon illumination lamp, and Applied Spectral software
• Arcturus Laser Capture Microdissection system, ideal for precise, under-microscope collection of tissue cells for DNA/RNA analysis
  • Compact system incorporating an inverted microscope for direct visualization of the cells of interest, an IR diode laser and a UV laser; see specifications

Image processing workstations/computers

• Linux workstation with Softworx software for image processing & deconvolution of the data generated on the DeltaVision microscopes.
• Mac Power G5 computer with Volocity/OpenLab software for image processing and for facilitating data transfer from the Unix based imaging systems.
• PC dual core with Imaris, MicroView and Live software for image processing and for facilitating data transfer from and to Window based imaging systems.

Sample preparation

• Edwards Vacuum Deposition System for metal and carbon coating of electron microscopy samples.
• Tousimis Samdri-795 Critical Point Drier for sample preparation for electron microscopy.