Instrumentation

Below is the basic information for all instruments in the Microscopy and Imaging Facility. Additional detailed and up-to-date information regarding instrument capabilities can be found here.

Fluorescence and Bright Field Microscopes

  • Applied Precision DeltaVision Spectris Imaging System, best for live/fixed thin samples
    • Inverted Olympus X71microscope with XYZ nano-motion stage, Solent environment chamber, Mercury lamp illumination
    • Softworx deconvolution software
  • Applied Precision DeltaVision Microscope with Ultimate Focus and TIRF module, best for live cell imaging, photokinetics experiments and TIRF imaging
    • Inverted Olympus X71 microscopes with XYZ nano-motion stage, environment chamber, Solid State Illumination module, four (4) lasers, Ultimate Focus, TIRF module
    • Softworx deconvolution software
  • Applied Precision DeltaVision-OMX Super-Resolution Microscope, best for imaging thin samples
    • Experimental microscope (OMX) with XYZ nano-motion stage, Structured Illumination, three (3) lasers, three cameras, heated stage
    • Softworx processing software
  • Nikon Spinning-disk Confocal Microscope with TIRF module, best for live imaging/thick samples and for live TIRF imaging
    • Nikon inverted microscope with XYZ stage, with lateral arm Yokogawa spinning disk and back illumination TIRF module, environment chamber, laser (3 lines) / 7 Wavelength Solid State Illumination
    • Nikon NIS Elements software
  • Nikon 1AR Ultra-Fast Spectral Scanning Confocal Microscope, best for imaging live/fixed thick samples
    • Nikon inverted microscope with XYZ stage & environment chamber, a four standard detector unit and a 32 ultra fast spectral (resonant scanning) detector unit, 3 lasers (7 lines) and Lumen lllumination Source
    • Nikon NIS Elements software
  • Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope, best for imaging live/fixed thick samples
    • Olympus inverted IX81 microscope with heated & CO2 stage incubator, MaiTai laser, photomultiplier detectors -2 channels
    • Olympus software.
  • Zeiss microscope, good for imaging fixed thin samples and histology
    • Zeiss Axioplan II upright microscope with monochrome and color cameras, Mercury lamp illumination
    • OpenLab / Volocity software

Specialty Microscopes

Image processing workstations/computers

  • Linux workstation with Softworx software for image processing & deconvolution of the data generated on the DeltaVision microscopes.
  • Mac Power G5 computer with Volocity/OpenLab software for image processing and for facilitating data transfer from the Unix based imaging systems.
  • PC dual core with Imaris, MicroView and Live software for image processing and for facilitating data transfer from and to Window based imaging systems.

Sample preparation

  • Edwards Vacuum Deposition System for metal and carbon coating of electron microscopy samples.
  • Tousimis Samdri-795 Critical Point Drier for sample preparation for electron microscopy.