Instrumentation

Below is the basic information for all instruments in the Microscopy Core Facility. Additional detailed and up-to-date information regarding instrument capabilities can be found here.

Fluorescence and Bright Field Microscopes

  • Applied Precision DeltaVision Spectris Imaging System, best for live/fixed thin samples
    • Inverted Olympus X71microscope with XYZ nano-motion stage, Solent environmental chamber, Mercury lamp illumination
    • Softworx deconvolution software
  • Applied Precision DeltaVision Microscope with Ultimate Focus and TIRF module, best for live cell imaging, photokinetics experiments and TIRF imaging
    • Inverted Olympus X71 microscopes with XYZ nano-motion stage, environmental chamber, solid state illumination module, four lasers, Ultimate Focus, TIRF module
    • Softworx deconvolution software
  • Applied Precision DeltaVision-OMX Super-Resolution Microscope, best for imaging thin samples
    • Experimental microscope (OMX) with XYZ nano-motion stage, Structured Illumination, three lasers, three cameras, heated stage
    • Softworx processing software
  • Nikon Spinning-disk Confocal Microscope with TIRF module, best for live imaging/thick samples and for live TIRF imaging
    • Nikon inverted microscope with XYZ stage, left lateral arm Yokogawa spinning disk module and Andor Clara camera, right lateral arm wide field epifluorescence module, transmitted light detection and Photometrics camera, back illumination TIRF module, environmental chamber, four lasers, seven line solid state illumination source
    • Nikon NIS Elements software
  • Nikon A1R Ultra-Fast Spectral Scanning Confocal Microscope, best for imaging live/fixed thick samples
    • Nikon inverted microscope with XYZ stage and environmental chamber, left lateral arm scanning head with a four standard detector unit and a 32 ultra fast spectral (resonant scanning) detector unit, four lasers, seven line solid state illumination source, and right lateral arm wide field epifluorescence module, transmitted light detection and Andor Clara camera, and Lumen illumination source
    • Nikon NIS Elements software
  • Olympus FV1000 Multiphoton Laser Scanning Confocal Microscope, best for imaging live/fixed thick samples and intravital imaging
    • Olympus inverted IX81 microscope with heated and CO2 stage incubator, MaiTai laser, photomultiplier detectors -four channels
    • Olympus software
  • Olympus FV1200 Laser Scanning Confocal Microscope, best for imaging live/fixed thick samples
    • Olympus inverted IX83 microscope with heated and CO2 stage incubator, five lasers, transmitted light detection, three PMTs and two high sensitivity detectors
    • Olympus software
    • Hamamatsu ImagEM high sensitivity camera
  • Zeiss microscope, good for imaging fixed thin samples and histology
    • Zeiss Axioplan II upright microscope with monochrome and color cameras, Mercury lamp illuminatio
    • OpenLab / Volocity software

Specialty Microscopes

Image processing workstations/computers

  • Linux workstation with Softworx software for image processing and deconvolution of the data generated on the DeltaVision microscopes.
  • PC dual core with Volocity, NIS Elements, ImageJ and Imaris software for image processing and for facilitating data transfer from and to Windows-based imaging systems

Sample preparation

  • Edwards Vacuum Deposition System for metal and carbon coating EM samples
  • Tousimis Samdri-795 Critical Point Drier for EM sample