Pricing

Please note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Current rates effective Feb 2013.

Each service is described in detail on the Services page.

Starting Monday September 17, 2012, the ES Cell & Transgenics Core is excited to start using iLab, an online system to streamline the process of ordering and billing for core service requests. Information is available on the Forms page.

service	description	MIT-KI	MIT (non-KI)*
Gene Targeting in ES cells	Gene targeting with 240-300 colonies picked	$2000	$3950
	Small targeting, picking 96 colonies or fewer	$1450	$2700
	Assistance with transgenic vector design	FREE	FREE
	Please start by reading our "Beginner’s Guide to Gene Targeting" on Methods

Microinjection	Injection of DNA, ES cells, or virus into pre-implantation, diploid embryos (C57BL6 or Balb/c)	$1350	$1750
	Injection of F1 hybrid ES cells into CD-1 tetraploid blastocysts	$2010	$2700
	Fee for each additional recipient mouse A surcharge will be added to the cost for investigators who do not use standard strains of mice for donors. The surcharge will cover the purchase cost of the mice as well as per diem and colony management.	$50	$50
	Culture of ES cells and preparation for blastocyst injection	$180	$255
Karyotyping	Prepare and Ship 1 or 2 mouse ES lines for Karyotyping at CLG Each additional line added to a shipment to CLG (Cell Line Genetics charges $475 per line for karyotyping)	$250 $100
Pathogen Tests	ES cells imported from outside MIT will be expanded and submitted to DCM (DCM charge of $470 for testing at RADIL, not included)	$180	$255

Mice	Repository Mouse Strains expressing tissue-specific Cre, Flp or EGFP as well as controls. See Repository Mice Available.	varies by strain

Training	Training options include, but are not limited to: Generation of MEFs Isolation of mouse embryos Isolation and culture of blastocysts Generation of ES cell lines Culture of ES cells Differentiation of ES cells to specific tissue type Optimizing breeding conditions for mice	$55/hr	$82.50/hr

Reagents	Vials of 3x10'6 irradiated MEFs for use as feeders	$10	$15
	Vial of p1 MEFs	$50	$75
	Vial of germline-proven ES cells	$250	$375
	Media, Serum, and ESGRO for ES cell culture	at cost

Other services such as cryopreservation of sperm and embryos, IVF, and genotyping are also available. Please see the DCM website (Certificates Required) for more details.

* Due to parameters established by our funding, we are NOT able to provide any services to non-MIT investigators. If you are interested, the following Transgenic Facilities will provide targeting in ES cells for other academic institutions:
Baylor | Emory | Stanford