Please note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Current rates effective Aug 31, 2015.
Each service is described in detail on the Services page.
The ES Cell & Transgenics Core uses iLab, an online system, to streamline the process of requesting and billing core services. Information on registering for an iLab account is available on the Core Access page.
MIT (non-KI) & outside academic*
|Gene Targeting in ES cells||Gene targeting with 240-288 colonies isolated for screening||$2000||$3950|
|Small targeting, isolating 96 or fewer colonies||$1450||$2700|
|Assistance with transgenic vector design||FREE||$82.50/hr|
|Please start by reading our "Beginner’s Guide to Gene Targeting" on Methods|
Injection of DNA (BAC, Transgenic or CRISPR), RNA or virus into pronucleus of fertilized mouse eggs (C57BL6 or Balb/c)
Requests for Pronucler injection (PNI) of DNA, RNA or virus are processed by DCM. Please see the DCM website for the forms to request these services. (MIT Certificates Required)
|For CRISPR injections, 20ug of Cas9 mRNA||$60||$90|
|Injection of ES cells into pre-implantation, diploid blastocysts (C57BL6 or Balb/c)||$1420||$2000|
|Injection of F1 hybrid ES cells into CD-1 tetraploid blastocysts||$2110||$2836|
|Culture of ES cells and preparation for their injection into blastocysts||$180||$255|
|Karyotyping||Prepare and Ship 1 or 2 mouse ES lines for Karyotyping at Cell Line Genetics (CLG)||$300||$450|
|Each additional line added to a shipment to CLG||$100||$150|
|(Cell Line Genetics charges $450 per line for karyotyping)|
|Pathogen Tests||ES cells imported from outside MIT will be expanded and submitted (Charge of $470 for testing at IDEXX, not included)||$180||$255|
Pathogen-free mice are available for transfer to MIT Animal Facilities only, not to other institutions.
All mice are also availiable from The Jackson Laboratories. See Repository Mice Available page for details, including JAX strain#'s.
TPA-/- (for NeorR MEFs) $100/$150
|Training and Assisted Work||
Training options include, but are not limited to:
Culture of ES cells
Generation of MEFs
Isolation of mouse eggs or embryos
|Reagents||Vials of 3x10'6 irradiated MEFs for use as feeders||$10||$15|
|Vial of p2 NeoR MEFs||$50||$75|
|Vial of p2 DR4 MEFs (HygroR - PuroR- NeoR)||$100||$150|
|Vial of germline-proven ES cells||$300||$450|
|Media, Serum, and ESGRO for ES cell culture||at cost|
Other services such as cryopreservation of sperm and embryos, IVF, and genotyping are also available from DCM.
Please see the DCM website (Certificates Required) for more details.
*As workflow permits, we do provide services to non-MIT academic institutions and for-profit researchers.