| Gene Targeting in ES Cells | ||
|---|---|---|
| Gene targeting with 240-288 colonies isolated for screening | $4,187 | $8,374 |
| Small targeting, isolating 96 or fewer colonies | $2,721 | $5,442 |
| Transfection of cell lines | $350 | $700 |
| Microinjection | ||
| Blastocyst harvest | $158 | $316 |
| Culture of ES cells and preparation for their injection into blastocysts | $275 | $550 |
| Preparation of cocktail containing Cas9/sgRNA complex and HDR DNA for injection into zygotes | $232 | $464 |
| Expansion and stocking a cell line or clone | $215 | $430 |
| Karyotyping | ||
| Prepare and ship 1 or 2 mouse ES lines for karyotyping at Cell Line Genetics (CLG) | $386 | $772 |
| Each additional line added to a shipment to CLG | $116 | $232 |
| Karyotyping (CLG charge) | $450 | $450 |
| Pathogen Tests | ||
| Preparation for testing | $215 | $430 |
| Testing at IDEXX (billed by vendor) | $805 | $805 |
| Cells that will be placed in a rodent must be free from known pathogens. Cell lines will be expanded according to testing requirements and submitted. | ||
| Mice | ||
|
C57B6L/6j: per mouse Pathogen-free mice are available for transfer to MIT Animal Facilities only, not to other institutions. All mice are also available from The Jackson Laboratories. See Repository Mice Available on Services page for details. |
$45.00 | $52.00 |
| Reagents | ||
|
Vials of 3x105 irradiated NeoR MEFs for use as feeders. One vial covers one multiwell plate or one 10cm dish. |
$24.00 | $36.00 |
| Vial of germline-proven ES cells | $455.00 | $682.50 |
| ES media w/LIF, 500mL | $238.00 | $357.00 |
| ES media w/LIF, 250mL | $143.00 | $214.50 |
| ES media w/LIF &G418, 250mL | $166.00 | $249.00 |
| ES media, no LIF, 500mL | $169.00 | $253.50 |
| Freezing media, 50mL | $80.00 | $120.00 |
| ES-grade 1% Gelatin, 500mL | $41.00 | $61.50 |
| ES-grade PBS, 500mL | $19.00 | $28.50 |
| 0.25% Trypsin, 100mL | $19.00 | $28.50 |
| Matrigel, 500uL | $18.00 | $27.00 |
| Other Services | ||
| Consultation and project management | $128 | $256 |
|
Assisted work, including: Culture of mouse embryonic stem (ES) cells and organoids Generation of new cell lines: ES cells, MEFs, mouse intestinal organoids or other primary cell cultures (all mammals) Stable transfection of cell lines to introduce elements such as reporters Isolation of mouse eggs, blastocysts or embryos Optimizing breeding conditions for mice |
$120 | $240 |
| Training: includes options listed for assisted work and more | $89 | $178 |
| Equipment use (Nucleofector, GenePulser, Nanoview) | $14.50 | $29 |
The above fee schedule is effective August 1, 2023.
Please note: rates are set periodically, based on operating costs and in accordance with MIT and NCI policies. They are reviewed and approved annually by the MIT Office of Cost Analysis. Services are billed at the rate listed on the date of service delivery.