The documents listed below are detailed protocols for the new and experienced ES cell/mouse user alike. These protocols have been tested and optimized repeatedly, and should provide an efficient means to reproducible results. Some protocols include MIT-specific information, though all should be applicable in general.
If you have a protocol that would be useful for other users, please contact Aurora (firstname.lastname@example.org) and we'd be delighted to add it to this site. Also, if you have any questions about any part of a protocol, please let Aurora know. We strive to provide clear, easy-to-use protocols to reduce the hassle and mystery involved with ES cell culture, gene targeting and mouse models. Your feedback helps us reach that goal.
There are more protocols and reagent information for ES cell culture to be found under Useful Links.